Co-design of Reconfigurable and Multifunction Passive RF/Microwave Components

In order to meet the market demands, multi-band communication systems that are able to accommodate different wireless technologies to be compatible with different wireless standards should be investigated and realized. Multifunction and multi-band RF front-end components are promising solutions for reducing the size and enhancing the performance of multi-band communication systems. This dissertation focuses on the design and implementation of different multifunction and tunable microwave components for use in multi-standard, flexible transceiver. For frequency-domain duplexing (FDD) communication systems, in which the uplink and downlink channels are carried on different RF frequencies, a diplexer is an essential component to separate the transmitting and receiving signals from the antenna. Electrically tunable diplexers simplify the architecture of reconfigurable RF-front end. Moreover, in modern communication systems, the crowding of the spectrum and the scaling of electronics can result in higher common-mode interference and even-order non-linearity issues. In this dissertation, three tunable compact SIW-based dual-mode diplexers, with various SE (single-ended) and BAL (balanced) capabilities, are introduced for the first time. The dual-mode operation results in a dependent tuning between the two ports. The presented designs are for SE-SE, SE-BAL, and BAL-BAL. However, based on the presented design concepts, any combination of the diplexer ports can be achieved in terms of supporting the balanced and single-ended system interface. The fabricated diplexers show low insertion loss, high isolation, good tuning range and high common mode rejection. Tunable bandstop filter (BSF) is one of the essential components in the design of RF front-ends that require wide-band operations. A wide-open front-end leaves the receiver vulnerable to jamming by high-power signals. As a result, this type of front-ends requires dynamic isolation of any interfering signal. Realization of such filters in a balanced configuration, as a second function, is an important step in the realization of full-balanced RF front-ends. Balanced (differential) circuits have many important advantages over unbalanced (single-ended) circuits such as immunity to system noise, reduction of transient noise generation and inherent suppression of even-order nonlinearities. All reported balanced filters are bandpass filters that target wide pass-bands and high common-mode rejection. These filters are necessary for wide-band RF front-ends but, as mentioned above, leave the system open to interferers and jammers. In this dissertation, a new differential coupling structure for evanescent-mode cavity resonators is developed, enabling the design of fully-balanced tunable BSF. The proposed filter is tunable from 1.57-3.18 GHz with 102% tuning range. In addition, over the full range, the measured 10-dB fractional bandwidth ranges from 1-2.4%, and the attenuation level is better than 47 dB. Lastly, Substrate Integrated Waveguide (SIW) evanescent-mode cavity resonators (EVA) are employed in the design of RF couplers, quadrature hybrid and rat-race couplers. These couplers are used in the design of numerous RF front-end components such as power amplifiers, balanced mixers, and antenna array feeding networks. Utilizing such resonators (EVA) in the design allows the couplers to have wide spurious-free range, low power consumption, high power handling capability and both tunability and filtering capabilities. The proposed quadrature hybrid coupler can be tuned starting from 1.32–2.22 GHz with a measured insertion loss range from 1.29 to 0.7 dB. The measured reflection and isolation are better than 12 dB and 17 dB, respectively. Moreover, the coupler has a measured spurious free range of 5.1–3fo (lowest–highest frequency). Regarding rat-race coupler, two designs are introduced. The first design is based on a full-mode cavity while the second one is more compact and based on a half-mode cavity. Both designs show more than 70% tuning range, and the isolation is better than 30 dB

Co-Design of Reconfigurable and Multifunction Passive RF/microwave Components

In order to meet the market demands, multi-band communication systems that are able to accommodate different wireless technologies to be compatible with different wireless standards should be investigated and realized. Multifunction and multi-band RF front-end components are promising solutions for reducing the size and enhancing the performance of multi-band communication systems. This dissertation focuses on the design and implementation of different multifunction and tunable microwave components for use in multi-standard, flexible transceiver. For frequency-domain duplexing (FDD) communication systems, in which the uplink and downlink channels are carried on different RF frequencies, a diplexer is an essential component to separate the transmitting and receiving signals from the antenna. Electrically tunable diplexers simplify the architecture of reconfigurable RF- front end. Moreover, in modern communication systems, the crowding of the spectrum and the scaling of electronics can result in higher common-mode interference and even-order non-linearity issues. In this dissertation, three tunable compact SIW-based dual-mode diplexers, with various SE (single-ended) and BAL (balanced) capabilities, are introduced for the first time. The dual-mode operation results in a dependent tuning between the two ports. The presented designs are for SE-SE, SE-BAL, and BAL-BAL. However, based on the presented design concepts, any combination of the diplexer ports can be achieved in terms of supporting the balanced and single-ended system interface. The fabricated diplexers show low insertion loss, high isolation, good tuning range and high common mode rejection. Tunable bandstop filter (BSF) is one of the essential components in the design of RF front-ends that require wide-band operations. A wide-open front-end leaves the receiver vulnerable to jamming by high-power signals. As a result, this type of front-ends requires dynamic isolation of any interfering signal. Realization of such filters in a balanced configuration, as a second function, is an important step in the realization of full-balanced RF front-ends. Balanced (differential) circuits have many important advantages over unbalanced (single-ended) circuits such as immunity to system noise, reduction of transient noise generation and inherent suppression of even-order nonlinearities. All reported balanced filters are bandpass filters that target wide pass-bands and high common-mode rejection. These filters are necessary for wide-band RF front-ends but, as mentioned above, leave the system open to interferers and jammers. In this dissertation, a new differential coupling structure for evanescent-mode cavity resonators is developed, enabling the design of fully-balanced tunable BSF. The proposed filter is tunable from 1.57-3.18 GHz with 102% tuning range. In addition, over the full range, the measured 10-dB fractional bandwidth ranges from 1-2.4%, and the attenuation level is better than 47 dB. Lastly, Substrate Integrated Waveguide (SIW) evanescent-mode cavity resonators (EVA) are employed in the design of RF couplers, quadrature hybrid and rat-race couplers. These couplers are used in the design of numerous RF front-end components such as power amplifiers, balanced mixers, and antenna array feeding networks. Utilizing such resonators (EVA) in the design allows the couplers to have wide spurious-free range, low power consumption, high power handling capability and both tunability and filtering capabilities. The proposed quadrature hybrid coupler can be tuned starting from 1.32-2.22 GHz with a measured insertion loss range from 1.29 to 0.7 dB. The measured reflection and isolation are better than 12 dB and 17 dB, respectively. Moreover, the coupler has a measured spurious free range of 5.1-3f_o (lowest-highest frequency). Regarding rat-race coupler, two designs are introduced. The first design is based on a full-mode cavity while the second one is more compact and based on a half-mode cavity. Both designs show more than 70% tuning range, and the isolation is better than 30 dB

Immunogenicity and Cross-Protective Efficacy Induced by an Inactivated Recombinant Avian Influenza A/H5N1 (Clade 2.3.4.4b) Vaccine against Co-Circulating Influenza A/H5Nx Viruses

Controlling avian influenza viruses (AIVs) is mainly based on culling of the infected bird flocks or via the implementation of inactivated vaccines in countries where AIVs are considered to be endemic. Over the last decade, several avian influenza virus subtypes, including highly pathogenic avian influenza (HPAI) H5N1 clade 2.2.1.2, H5N8 clade 2.3.4.4b and the recent H5N1 clade 2.3.4.4b, have been reported among poultry populations in Egypt. This demanded the utilization of a nationwide routine vaccination program in the poultry sector. Antigenic differences between available avian influenza vaccines and the currently circulating H5Nx strains were reported, calling for an updated vaccine for homogenous strains. In this study, three H5Nx vaccines were generated by utilizing the reverse genetic system: rgH5N1_2.3.4.4, rgH5N8_2.3.4.4 and rgH5N1_2.2.1.2. Further, the immunogenicity and the cross-reactivity of the generated inactivated vaccines were assessed in the chicken model against a panel of homologous and heterologous H5Nx HPAIVs. Interestingly, the rgH5N1_2.3.4.4 induced high immunogenicity in specific-pathogen-free (SPF) chicken and could efficiently protect immunized chickens against challenge infection with HPAIV H5N1_2.3.4.4, H5N8_2.3.4.4 and H5N1_2.2.1.2. In parallel, the rgH5N1_2.2.1.2 could partially protect SPF chickens against infection with HPAIV H5N1_2.3.4.4 and H5N8_2.3.4.4. Conversely, the raised antibodies to rgH5N1_2.3.4.4 could provide full protection against HPAIV H5N1_2.3.4.4 and HPAIV H5N8_2.3.4.4, and partial protection (60%) against HPAIV H5N1_2.2.1.2. Compared to rgH5N8_2.3.4.4 and rgH5N1_2.2.1.2 vaccines, chickens vaccinated with rgH5N1_2.3.4.4 showed lower viral shedding following challenge infection with the predefined HPAIVs. These data emphasize the superior immunogenicity and cross-protective efficacy of the rgH5N1_2.3.4.4 in comparison to rgH5N8_2.3.4.4 and rgH5N1_2.2.1.2

Emergence of Highly Pathogenic Avian Influenza A Virus (H5N1) of Clade 2.3.4.4b in Egypt, 2021–2022

Wild migratory birds have the capability to spread avian influenza virus (AIV) over long distances as well as transmit the virus to domestic birds. In this study, swab and tissue samples were obtained from 190 migratory birds during close surveillance in Egypt in response to the recent outbreaks of the highly pathogenic avian influenza (HPAI) H5N1 virus. The collected samples were tested for a variety of AIV subtypes (H5N1, H9N2, H5N8, and H6N2) as well as other pathogens such as NDV, IBV, ILT, IBDV, and WNV. Among all of the tested samples, the HPAI H5N1 virus was found in six samples; the other samples were found to be negative for all of the tested pathogens. The Egyptian HPAI H5N1 strains shared genetic traits with the HPAI H5N1 strains that are currently being reported in Europe, North America, Asia, and Africa in 2021–2022. Whole genome sequencing revealed markers associated with mammalian adaption and virulence traits among different gene segments, similar to those found in HPAI H5N1 strains detected in Europe and Africa. The detection of the HPAI H5N1 strain of clade 2.3.4.4b in wild birds in Egypt underlines the risk of the introduction of this strain into the local poultry population. Hence, there is reason to be vigilant and continue epidemiological and molecular monitoring of the AIV in close proximity to the domestic–wild bird interface

A longitudinal study of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels

Abstract Background Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in humans in 2012. Since then, 2605 cases and 937 associated deaths have been reported globally. Camels are the natural host for MERS-CoV and camel to human transmission has been documented. The relationship between MERS-CoV shedding and presence of neutralizing antibodies in camels is critical to inform surveillance and control, including future deployment of camel vaccines. However, it remains poorly understood. The longitudinal study conducted in a closed camel herd in Egypt between December 2019 and March 2020 helped to characterize the kinetics of MERS-CoV neutralizing antibodies and its relation with viral shedding. Results During the 100-day longitudinal study, 27 out of 54 camels (50%) consistently tested negative for presence of antibodies against MERS-CoV, 19 (35.2%) tested positive and 8 (14.8%) had both, positive and negative test results. Fourteen events that could be interpreted as serological indication of probable infection (two seroconversions and twelve instances of positive camels more than doubling their optical density ratio (OD ratio) in consecutive samples) were identified. Observed times between the identified events provided strong evidence (p = 0.002) against the null hypothesis that they occurred with constant rate during the study, as opposed to clustering at certain points in time. A generalized additive model showed that optical density ratio (OD ratio) is positively associated with being an adult and varies across individual camels and days, peaking at around days 20 and 90 of the study. Despite serological indication of probable virus circulation and intense repeated sampling, none of the tested nasal swab samples were positive for MERS-CoV RNA, suggesting that, if the identified serological responses are the result of virus circulation, the virus may be present in nasal tissue of infected camels during a very narrow time window. Conclusions Longitudinal testing of a closed camel herd with past history of MERS-CoV infection is compatible with the virus continuing to circulate in the herd despite lack of contact with other camels. It is likely that episodes of MERS-CoV infection in camels can take place with minimal presence of the virus in their nasal tissues, which has important implications for future surveillance and control of MERS-CoV in camel herds and prevention of its zoonotic transmission